In Vitro Assessment of Emd Based Biomaterial Biocompatibility

Tissue engineering is a rapidly developing area that might restore, maintain or improve tissue functions. The major elements of tissue engineering are: integrate cells, scaffolds and biologically active molecules. These components act synergistically to regulate stem cell propagation and differentiation thus ensuring tissue regeneration. For evaluation of biocompatibility of enamel matrix derivative (EMD) based biomaterial, palatal mesenchymal stem (PMSCs) cells at a density of 1x10 cells were co cultured. The level of adherence was daily assessed. The viability of cells was evaluated after 24h, 72h and 7 day using FDA (fluorescein diacetate) assay. Our study revealed that the selected biomaterial are biocompatible and can be used as scaffolds for mesenchymal stem cells delivery especially for periodontal regeneration.